mouse vasoactive intestinal peptide vip elisa kit  (Elabscience Biotechnology)

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: External damp environment aggravates diarrhea in spleen deficiency and dampness syndrome in mice: involvement of small intestinal contents microbiota, energy metabolism, gastrointestinal and fluid functions

doi: 10.3389/fcimb.2024.1495311

Figure Lengend Snippet: Differences in gastrointestinal function and fluid metabolism in diarrhea patients with spleen deficiency and dampness. (A) Serum D-xylose levels. (B) Serum gastrin (Gas) levels. (C) Serum vasoactive intestinal peptide (VIP) levels. Statistical significance is indicated as follows: ** p <0.01,*** p <0.001. The experimental groups included ZC (normal group), ZL (standing group), ZLZ (standing combined with lard group), and ZLZS (standing combined with internal and external dampness group).

Article Snippet: The specifications for the ELISA kits used in the analysis are as follows: Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit (Batch number: JM-02729M2, Jiangsu Jingmei Biotechnology Co., Ltd.), Mouse Gastrin ELISA Kit (Batch number: JM-03140M2, Jiangsu Jingmei Biotechnology Co., Ltd.), Mouse D-xylose ELISA Kit (Batch number: JM-12962M2, Jiangsu Jingmei Biotechnology Co., Ltd.), Mouse Cyclic Guanosine Monophosphate (cGMP) ELISA Kit (Batch number: JM-02304M2, Jiangsu Jingmei Biotechnology Co., Ltd.), and Mouse Cyclic Adenosine Monophosphate (cAMP) ELISA Kit (Batch number: JM-02827M2, Jiangsu Jingmei Biotechnology Co., Ltd.).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Vasoactive intestinal peptide promotes secretory differentiation and mitigates radiation-induced intestinal injury

doi: 10.1186/s13287-024-03958-z

Figure Lengend Snippet: VIP mitigates irradiation-induced injury in vivo. A Experimental outline and treatment scheme for VIP or water (IR control) administration in mice following acute abdominal irradiation, compared with sham-irradiated untreated (Sham Control) mice. B , ELISA analysis of VIP levels in the intestinal wall on day 6 following acute abdominal irradiation. n = 5 (Sham Control), n = 7 (IR Control). C , ELISA analysis of TNF-alpha levels in the intestinal wall of sham-irradiated and irradiated mice in the absence or presence of VIP. n = 7 (Sham Control), n = 6 (IR Control; IR VIP). D Pathological scoring of irradiated mouse tissue in the absence or presence of VIP and representative H&E images (Scale bars = 100 µm), n = 5 per group. E, Immunofluorescent analysis of LYZ1 staining in jejunal tissue from irradiated mice in the absence or presence of VIP (Scale bars = 100 µm). n = 7 (Sham Control; IR Control), n = 8 (IR VIP). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are shown as means ± SEM, n = number of biological replicates. Statistical analysis was performed by Student’s t -test in ( B , D ) and one-way ANOVA with Tukey's multiple comparisons test in ( C , E )

Article Snippet: VIP and TNF-alpha levels were measured using Mouse VIP (Competitive EIA) ELISA Kit (LSBio, #LS-F4059) and Mouse TNF Alpha (Sandwich ELISA) ELISA Kit (LSBio, #LS-F5192), respectively, according to the manufacturer’s instructions.

Techniques: Irradiation, In Vivo, Control, Enzyme-linked Immunosorbent Assay, Staining

Journal: Frontiers in Nutrition

Article Title: Supplementation of Crataegi fructus alleviates functional dyspepsia and restores gut microbiota in mice

doi: 10.3389/fnut.2024.1385159

Figure Lengend Snippet: Hawthorn relieves symptoms of FD in mice. (A1) Body weight,♀; (A2) body weight,♂; (B1) food intake,♀; (B2) food intake,♂; (C1) water consumption,♀; (C2) water consumption,♂; (D) small intestinal propulsion rate; (E) intragastric residual rate; serum NO (F) and GAS (G) levels; VIP (H) , SP (I) , and 5-HT (J) levels in the duodenum. Data are presented as mean ± SD ( n = 10). * p < 0.05, ** p < 0.01.

Article Snippet: The 5-hydroxytryptamine (5-HT) ELISA Kit (Wuhan CUSABIO Co., Ltd., L211129838), the Mouse Substance P (SP) ELISA Kit (Wuhan CUSABIO Co., Ltd., L211012768), and the Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit (Wuhan CUSABIO Co., Ltd., L220113396) were used to determine the 5-HT, SP, and VIP levels in the duodenum.

Techniques:

Journal: Immunity, inflammation and disease

Article Title: Vasoactive intestinal peptide exerts therapeutic action by regulating PTEN in a model of Sjögren's disease.

doi: 10.1002/iid3.936

Figure Lengend Snippet: FIGURE 3 Vasoactive intestinal peptide (VIP) upregulated PTEN, VIP receptor mRNA expression, and Treg‐related cytokines in spleen. Q‐PCR results for VIP mRNA, VIP receptors mRNA expression (A), and PTEN/PI3K/AKT pathway (B) in the spleen. The concentrations of VIP in the serum (C) were measured using ELISA kits (n = 7). The abundance of interleukin (IL)‐10 (D) and IL‐2 (E) in the splenic tissue homogenate supernatant was determined by ELISA as well (n = 7). Unpaired Student t‐test was used in the analysis. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Serum extraction method as published,20 VIP levels in serum were measured using the mouse VIP ELISA kit (Elabscience).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Parasites & Vectors

Article Title: Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge

doi: 10.1186/s13071-022-05597-w

Figure Lengend Snippet: The designed immunization scheme and assay protocol. A total of three oral immunizations were given, and five mice of each group were euthanized for assays of intestinal sIgA and cytokines at weeks 0, 2, 4, 6, 7 and 11 after the first immunization. Serum-specific anti- Trichinella spiralis galectin (Tsgal) antibodies (total IgG, IgG1 and IgG2a) were measured by indirect enzyme-linked immunosorbent assay [ELISA] using recombinant Tsgal (rTsgal) at 2 weeks following each immunization and at weeks 1–5, respectively, following challenge. After being challenged with 300 T. spiralis muscle larvae, 10 mice of each group were sacrificed at weeks 7 and 11 after vaccination (i.e. 7 and 35 days post infection [dpi), and adults worms and muscle larvae were recovered to assess the protective efficacy of recombinant NC8-Tsgal/α-lactose against T. spiralis challenge. Histopathological changes of the intestine and muscles from infected mice were examined at 1 and 5 weeks after challenge (i.e. 7 and 35 dpi). Samples were collected at the time-points indicated in the figure. Ig, Immunoglobulin; IL, interleukin; IFN, interferon; sIgA, secretory (mucosal) IgA

Article Snippet: The levels of intestinal histamine concentrations were measured using a mouse ELISA kit according to the manufacturer's instructions (Elabscience Biotechnol, Wuhan, China).

Techniques: Indirect ELISA, Enzyme-linked Immunosorbent Assay, Recombinant, Infection, Muscles